Ebola virus outbreak toolbox
Updated | August 2024
Welcome to the Ebola virus Outbreak toolbox
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Key reference documents
- Ebola virus disease fact sheet (WHO, 2026)
- Ebola virus disease information page (WHO)
- Infection prevention and control guideline for Ebola and Marburg diseases (WHO, 2025)
- Ebola and Marburg virus disease epidemics: preparedness, alert, control and evaluation: Interim manual Version 1.2 (WHO, 2014)
Ongoing Bundibugyo virus disease outbreak
- Ongoing outbreak in the Democratic republic of Congo (AFRO, 2026)
- Ebola - The Democratic Republic of Congo, 2026 (WHO. 2026)
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Case definitions
These case definitions are taken from the Integrated disease surveillance and response technical guidelines: Booklet Two: sections 1, 2, and 3, 3rd ed (page 18).
Surveillance case definition
Suspected case
- any person presenting with onset of fever and no response to usual causes of fever in the area, AND at least one of the following signs: bloody diarrhoea, bleeding from gums, bleeding into skin (purpura), bleeding into eyes and urine.
Confirmed case
- a suspected case-patient with laboratory confirmation (positive IgM antibody, positive PCR or viral isolation), OR epidemiological link to confirmed cases or outbreak.
Alert case (community-based surveillance)
- any person presenting with onset of fever and no response to treatment of usual causes of fever in the area; OR
- at least one of the following signs: bleeding, bloody diarrhoea, bleeding into urine; OR
- any sudden death.
Outbreak case definitions
Suspected case
- any person, alive or dead, suffering or having suffered from sudden onset of high fever, and had contact with a suspected, probable, or confirmed Ebola case, or a dead or sick animal; OR
- any person with sudden onset of high fever, AND at least three of the following symptoms: headache, lethargy, anorexia/loss of appetite, aching muscles or joints, stomach pain, difficulty swallowing, vomiting, difficulty breathing, diarrhoea, hiccups; OR
- any person with inexplicable bleeding; OR
- any sudden, inexplicable death.
Probable case
- any suspected case-patient evaluated by a clinician, OR
- any deceased suspected case-patient (where it has not been possible to collect specimens for laboratory confirmation) having an epidemiological link with a confirmed case.
Confirmed case
- any suspected or probable case-patient with a positive laboratory result (detection of Ebola virus by reverse transcription polymerase chain reaction (RT-PCR), or detection of Immunoglobulin M (IgM) antibodies directed against Ebola viruses).
Non case
- any suspected or probable case-patient with a negative laboratory result (showing no specific antibodies, ribonucleic acid (RNA) or specific detectable antigens) more than 72 hours after symptom onset.
Contact person definition
- any person having had direct physical contact with the bodily fluids of a sick or deceased person or indirect contact through contaminated surfaces, material, or equipment, such as needles or soiled clothes, within the past 21 day.
Case definitions are highly context dependant and may vary from on outbreak to the other.
Dead or sick animal contacts
- Any person having been exposed to a sick or dead animal, less than 21 days before the identification as a contact by surveillance teams, in at least one of the following ways:
- direct physical contact with the animal
- direct contact with the animal’s blood or body fluids (including through the carving up the animal, or consumption of raw bush meat)
Laboratory contacts
- Any person having been exposed to biological material in a laboratory, less than 21 days before the identification as a contact by surveillance teams, in at least one of the following ways:
- has had direct contact with specimens collected from suspected patients
- has had direct contact with specimens collected from suspected animal cases
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Data collection tools
- Case investigation forms
- Ebola or Marburg case investigation and recording sheet. In: Ebola and Marburg virus disease epidemics: preparedness, alert, control and evaluation, Interim version 1.2 (WHO, 2014) Annex 5
- Electronic tools: Go. Data Ebola outbreak template
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Laboratory confirmation
- Ebola and Marburg virus disease epidemics: preparedness, alert, control, and evaluation, Interim version 1.2 (WHO, 2014 (Chapter 4 and associated annexes)).
- Diagnostic testing for Ebola and Marburg virus diseases: interim guidance. (WHO, 2024).
- Disease Commodity Packages - Ebola virus disease, (WHO, 2018).
- How to safely collect blood samples by phlebotomy from patients suspected to be infected with filovirus.(Interim guidance 2014)
- How to safely collect oral swabs from deceased patients suspected to be infected with Ebola or Marburg. (Rapid advice guideline 2014)
- How to safely ship human blood samples from suspected EBOD cases within a country by road, rail and sea. (Interim guideline 2017). (Interim guideline 2017)
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Response tools and resources
- Contact tracing during an outbreak of Ebola virus disease (WHO Regional Office for Africa, 2014).
- Clinical management of patients with viral haemorrhagic fever: A pocket guide for front-line health workers: interim emergency guidance for country implementation (WHO, 2016).
- Optimized supportive care for Ebola disease: clinical management standard operating procedures (WHO, 2019)
- Guidelines for the management of pregnant and breastfeeding women in the context of Ebola disease (WHO, 2020)
- Clinical care for survivors of Ebola disease: interim guidance (WHO, 2016)
- Ebola disease treatment centres: facility design and construction standards for preparing for and responding to outbreaks (WHO, 2024)
- Essential Items Estimator Tool for health facilities in Ebola disease context
- VHF Global clinical platform
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Training
- Ebola diseases (Open WHO)
- Ebola: ePROTECT (an occupational health and safety briefing) (Open WHO courses).
What is Integrated Outbreak Analytics and the CASS ? (The Democratic Republic of the Congo) (UNICEF, 2022)
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One Health resources
- Ebola virus disease (World Organisation for Animal Health)
- Frequently asked questions on Ebola virus disease (Food and Agriculture Organization of the United Nations, 2018)
- Animal health risk analysis: addressing Zaire Ebolavirus (EBOV) outbreaks (Food and Agriculture Organization of the United Nations, 2018)
- Investigating the role of bats in emerging zoonoses (Food and Agriculture Organization of the United Nations, 2011)
- Ebola and Marburg hemorrhagic fevers - Poster (World Organisation for Animal Health)
- Bush meat : prevention (World Organisation for Animal Health)
- Protecting wildlife, protecting ourselves. An outlook on the EBO-SURSY Project (World Organisation for Animal Health)